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  • Writer's pictureDr. C. S. Sada

What is IVF / Test Tube Baby?

IVF 'InVitro Fertilization' is a process in which egg from a woman's body is fertilized outside of her body and later the egg is put inside the same woman's uterus to develop further.


This is a very useful treatment to treat infertility. If you choose hope, everything is possible.

Below are some of the stages through which the IVF treatment is carried. The treatment is different for different type of couples who want to conceive with the help of IVF.


A thorough medical inspection and tests are carried to identify the infertility causes and then the treatment is suggested accordingly. Below are the stages through which the IVF treatment is carried.


1. Sperm Preparation


Sperm is usually provided the morning of the egg retrieval. Occasionally the sperm is collected ahead of time and frozen for use after the egg retrieval. We can obtain sperm from the male partner through normal ejaculation or through more advanced sperm retrieval procedures such as microepididymal sperm aspiration (MESA), or testicular sperm extraction (TESE).

If your partner will be providing a semen specimen on the day of the egg retrieval, we recommend abstaining from intercourse for at least two days prior to the IVF procedure but not more than 6 days. This will help to obtain optimal sperm quality for the IVF procedure. Donor sperm may also be used; it will be thawed and prepared by the laboratory staff the morning of the retrieval.


After the lab receives the semen, the andrologist will first rinse the sample with a special solution that removes dead sperm and cellular debris while also protecting the living sperm. The andrologist then takes one ml of the sperm solution and removes extra fluid by centrifuging, spinning the sperm cells through a special medium to bring the concentration up to 30,000 motile sperm per ml. This concentrated sperm solution is then used to inseminate the eggs by adding a very small quantity of the sperm to each drop of culture medium containing the eggs.

2. Ovarian Stimulation

3. Transvaginal Oocyte Removal

4. Oocyte or Embryo Preservation

5. Fertilization

6. Embryo Culture

7. Embryo Transfer

8. Pregnancy


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